Albert John Ketterman

Albert John Ketterman, Ph.D.,
Assoc. Prof.

Ph.D. (Biochemistry), University of Queensland, 1990
Ext. 1279
Email: albert.ket@mahidol.ac.th
Field of Research:Molecular and cellular enzymology

Consequences of phosphorylation/glutathionylation in a Parkinson’s disease model human neuronal cell. Parkinson’s disease (PD) is the second most common movement disorder, one of the most common neurodegenerative diseases worldwide and a progressive neurodegenerative disorder. This means that over time the affected patients see their disability increase and their quality of life deteriorate. The incidence of PD also appears to be age related, with prevalence increasing with age. The idiopathic PD symptoms result from the degeneration of dopaminergic neurons. The events that trigger the apoptotic response are the deleterious consequences that lead to PD pathogenesis. We are interested in elucidating the protein changes that occur in the cell that lead to PD. An aspect of growing interest is the enzymatic attachment of glutathione to the reactive cysteines of proteins, a reaction known as glutathionylation.Increasing evidence suggests that glutathionylation may play similar roles as phosphorylation in cellular responses, for example to oxidative stress. Currently more than 150 proteins have been identified as being glutathionylated. These proteins range from metabolic enzymes to proteins involved in signal transduction pathways. Glutathionylation of proteins appears to impact on the biological function of the affected protein. We are in the process of identifying proteins that are phosphorylated/glutathionylated in a human neuronal cell thereby affecting biological function which may ultimately lead to conditions producing Parkinson’s disease.

Selected Publication

1. Saisawang, C., Wongsantichon, J. and Ketterman*,A.J. (2012) A preliminary characterization of the cytosolic glutathione transferase proteome from Drosophilamelanogaster. Biochem. J. 442, 181-190.

2. Ketterman*,A.J., Saisawang, C. and Wongsantichon, J. (2011) Insect glutathione transferases. Drug Metab Rev43, 253-265.

3. Wongsantichon, J., Robinson, R.C. and Ketterman*,A.J. (2010) Structural contributions of delta class glutathione transferase active site residues to catalysis. Biochem. J. 428,25-32.

4. Wongsantichon, J. and Ketterman*, A.J. (2006) An Intersubunit Lock-and-Key ‘Clasp’ Motif in the Dimer Interface of Delta Class Glutathione Transferase.Biochem. J.394, 135-144.

5. Winayanuwattikun, P. and Ketterman*, A.J. (2005) An electron-sharing network involved in the catalytic mechanism is functionally conserved in different glutathione transferase classes. J. Biol. Chem.280, 31776-31782.

Full Publications

1. Wongtrakul, J., Janphen, K., Saisawang, C. and Ketterman*, A.J. (2014) Interaction of Omega, Sigma and Theta Glutathione Transferases with p38b MAP Kinase Protein from the fruit fly, Drosophila melanogaster. J. Insect Sci. 14, 60.

2. Bocedi, A., Fabrini, R., Farrotti, A., Stella, L., Ketterman, A. J., Pedersen, J. Z., Allocati, N., Lau, P. C., Grosse, S., Eltis, L. D., Ruzzini, A., Edwards, T. E., Morici, L., Del Grosso, E., Guidoni, L., Bovi, D., Lo Bello, M., Federici, G., Parker, M. W., Board, P. G., Ricci, G. (2013)  The impact of nitric oxide toxicity on the evolution of the glutathione transferase superfamily. A proposal for an evolutionary driving force. J. Biol. Chem.288, 24936-24947.

3. Wongsantichon, J., Robinson, R.C. and Ketterman*,A.J. (2012) Structural evidence for conformational changes of Delta class glutathione transferases after ligand binding. Arch. Biochem. Biophys. 521, 77-83.

4. Wongtrakul, J., Sukittikula, S., Saisawang, C. and Ketterman*, A.J. (2012) Mitogen-activated Protein (MAP) Kinase p38b Interaction with Delta Class Glutathione Transferases from the fruit fly Drosophila melanogaster. J. Insect Sci. 12, 107.

5. Saisawang, C., Wongsantichon, J. and Ketterman*,A.J. (2012) A preliminary characterization of the cytosolic glutathione transferase proteome from Drosophilamelanogaster. Biochem. J. 442, 181-190.

6. Ketterman*,A.J., Saisawang, C. and Wongsantichon, J. (2011) Insect glutathione transferases. Drug Metab Rev43, 253-265.

7. Wongsantichon, J., Robinson, R.C. and Ketterman*,A.J. (2010) Structural contributions of delta class glutathione transferase active site residues to catalysis. Biochem. J. 428,25-32.

8. Wongtrakul, J., Pongjaroenkit, S., Leelapat, P., Nachaiwieng, W., Prapanthadara, L. and Ketterman*, A.J. (2010) Expression and characterization of three new glutathione transferases, an epsilon (AcGSTE2-2), omega (AcGSTO1-1) and theta (AcGSTT1-1) from Anopheles cracens (Diptera: Culicidae) a major Thai malaria vector. J. Med Entomol. 47, 162-171.

9. Wongtrakul, J., Wongsantichon, J.,Vararattanavech, A., Leelapat, P.  Prapanthadara, L. and Ketterman*, A.J. (2009) Molecular cloning and expression of several new Anopheles cracensEpsilon class glutathione transferases. Protein and Peptide Lett.16, 75-81.

10. Lerksuthirat, T. and Ketterman*, A.J. (2008) Characterization of putative hydrophobic substrate binding site residues of a Delta class glutathione transferase from Anopheles dirus. Arch. Biochem. Biophys.479, 97-103.

11. Vararattanavech, A. and Ketterman*, A.J. (2007) A functionally conserved basic residue in glutathione transferases interacts with the glycine moiety of glutathione and is pivotal for enzyme catalysis. Biochem. J.406, 247-256.

12. Winayanuwattikun, P. and Ketterman*, A.J. (2007) Glutamate 64, a newly identified residue of the functionally conserved electron-sharing network contributes to catalysis and structural integrity of glutathione transferases.Biochem. J.402, 339-348.

13. Piromjitpong, J., Wongsantichon, J. and Ketterman*, A.J. (2007) Differences in the subunit interface residues of alternatively spliced glutathione transferases affects catalytic and structural functions. Biochem. J.401, 635-644.

14. Wongsantichon, J., Yuvaniyama, J. and Ketterman*, A.J. (2006) Crystallization and preliminary X-ray crystallographic analysis of a highly stable mutant V107A of glutathione transferase from Anopheles dirus in complex with glutathione. ActaCrys. F 62, 310-312.

15. Laohavechvanich, P., Kangsadalampai, K., Tirawanchai, N. and Ketterman, A.J. (2006) Effect of different Thai traditional processing of various hot chili peppers on urethane-induced somatic mutation and recombination in Drosophila melanogaster: assessment of the role of glutathione transferase activity. Food Chem. Toxicol.44, 1348-1354.

16. Charoensilp, G., Vararattanavech, A., Leelapat, P., Prapanthadara, L. and Ketterman*, A.J. (2006) Characterization of Anopheles dirus glutathione transferase Epsilon 4. ScienceAsia 32, 159-165.

17. Wongsantichon, J. and Ketterman*, A.J. (2006) An Intersubunit Lock-and-Key ‘Clasp’ Motif in the Dimer Interface of Delta Class Glutathione Transferase.Biochem. J.394, 135-144.

18. Vararattanavech, A., Prommeenate, P. and Ketterman*, A.J. (2006) The structural roles of a conserved small hydrophobic core in the active site and an ionic bridge in domain I of delta class glutathione transferase. Biochem. J.393, 89-95.

19. Bogoyevitch, M.A., Barr, R.K., Ketterman, A.J. (2005) Peptide inhibitors of protein kinases – discovery, characterisation and use. Biochim. Biophys. Acta -Proteins & Proteomics 1754,79-99.

20. Winayanuwattikun, P. and Ketterman*, A.J. (2005) An electron-sharing network involved in the catalytic mechanism is functionally conserved in different glutathione transferase classes. J. Biol. Chem.280, 31776-31782.

21. Wongsantichon, J. and Ketterman*, A.J. (2005) [6] Alternative Splicing Of Glutathione S-Transferases. Edited by Helmut Sies and Lester Packer. InMethods in Enzymology, “Phase II: Conjugation Enzymes, Glutathione Transferases and Transport Systems”. Vol. 401, 100-116. Elsevier Inc., San Diego.

22. Udomsinprasert, R., Pongjaroenkit, S., Wongsantichon, J., Oakley, A.J., Prapanthadara, L., Wilce, M.C.J.  andKetterman*, A.J. (2005) Identification, Characterization And Structure Of A New Delta Class Glutathione Transferase Isoenzyme. Biochem. J. 388, 763-771.

23. Wongtrakul, J., Sramala, I., Prapanthadara, L. and Ketterman*, A.J. (2005) Intra-subunit residue interactions from the protein surface to the active site of glutathione S-transferase AdGSTD3-3 impact on structure and enzyme properties. Insect Biochem. Molec. Biol.35, 197-205.

24. Udomsinprasert, R., Bogoyevitch, M.A. and Ketterman*, A.J. (2004) Reciprocal Regulation of Glutathione S-Transferase Spliceforms and the Drosophila c-Jun N-terminal Kinase Pathway Components. Biochem. J. 383,483-490.

25. Winayanuwattikun, P. and Ketterman*, A.J. (2004) Catalytic and structural contributions for glutathione binding residues in a delta class glutathione S-transferase. Biochem. J. 382,751-757.

26. Bogoyevitch, M.A., Boehm, I., Oakley, A., Ketterman, A.J. and Barr, R.K. (2004) Targeting the JNK MAPK cascade for inhibition: basic science and therapeutic potential. Biochim. BiophysActa1697, 89-101.

27. Wongtrakul, J., Udomsinprasert, R. and Ketterman*, A.J. (2003) Non-active site residues Cys69 and Asp150 affected the enzymatic properties of glutathione S-transferase AdGSTD3-3. Insect Biochem. Molec. Biol.33, 971-979.

28. Vararattanavech, A. and Ketterman*, A.J. (2003) Multiple roles of glutathione binding-site residues of glutathione S-transferase. Protein and Peptide Lett.,10, 441-448.

29. Wongsantichon, J., Harnnoi, T. and Ketterman*, A.J. (2003) A sensitive core region in the structure of glutathione S-transferases. Biochem. J.373, 759–765.

30. Wongtrakul, J., Sramala, I. and Ketterman*, A.J. (2003) A non-active site residue, cysteine 69, of glutathione S-transferase AdGSTD3-3 has a role in stability and catalytic function. Protein and Peptide Lett.,10, 375-385.

31. Udomsinprasert, R., and Ketterman*, A.J. (2002) Expression and Characterization of a Novel Class of Glutathione S-Transferase from Anopheles dirus. Insect Biochem. Molec. Biol.32: 425-433.

32. Huong, N.T., Sonthayanon, P., Ketterman, A.J., and Panyim, S. (2001) A rapid polymerase chain reaction based method for identification of the Anopheles dirus sibling species. Southeast Asian J. Trop. Med. Public Health32: 615-620.

33. Oakley, A.J., Harnnoi, T., Udomsinprasert, R., Jirajaroenrat, K., Ketterman, A.J., and Wilce, M.C.J. (2001) The crystal structures of glutathione S-transferases isozymes 1–3 and 1–4 from Anopheles dirusspecies B. Protein Sci.10: 2176-2185.

34. Oakley, A.J., Ketterman, A., and Wilce, M.C.J. (2001) Structural Biology and its Applications to the Health Sciences. Croat. Med. J. 42: 374-377.

35. Oakley, A.J., Jirajaroenrat, K., Harnnoi, T., Ketterman, A.J., and Wilce, M.C.J. (2001) Crystallization of two glutathione S-transferases from an unusual gene family. ActaCryst. D57: 870-872.

36. Jirajaroenrat, K., Pongjaroenkit, S., Krittanai, C., Prapanthadara, L., Ketterman*, A.J. (2001) Heterologous expression and characterization of alternatively spliced glutathione S-transferases from a single Anopheles gene. Insect Biochem. Molec. Biol. 31: 867-875.

37. Kaewsuk,,S., Hutamekalin,,P., Ketterman,,A.J., Khotchabhakdi,,N., Govitrapong,,P., Casalotti,S.O. (2001) Morphine induces short-lived changes in G-protein gene expression in rat prefrontal cortex. Eur. J. Pharmacol. 411: 11-16.

38. Pongjaroenkit, S., Jirajaroenrat, K., Boonchauy, C., Chanama, U., Leetachewa, S., Prapanthadara, L. and Ketterman*, A.J. (2001) Genomic organization and putative promoters of highly conserved glutathione S-transferases originating by alternative splicing in Anopheles dirus.   Insect Biochem. Molec. Biol.31: 75-85.

39. Ketterman*, A.J., Prommeenate, P., Boonchauy, C., Chanama, U., Leetachewa, S., Promtet, N., Prapanthadara, L. (2001) Single amino acid changes outside the active site significantly affect activity of glutathione S-transferases.   Insect Biochem. Molec. Biol. 31: 65-74.

40. Prapanthadara, L., Promtet, N., Koottathep, S.,  Somboon, P. and Ketterman, A.J. (2000) Isoenzymes of glutathione S-transferases from the mosquito Anopheles dirus species B:  The purification, partial characterization and interaction with various insecticides. Insect Biochem. Molec. Biol.30:395-403.

41. Prapanthadara,L.; Koottathep,S.; Promtet,N.; Suwonkerd,W.; Ketterman,A.J.; Somboon,P. (2000) Correlation of glutathione S-transferase and DDT dehydrochlorinase activities with DDT susceptibility in Anopheles and Culex mosquitos from northern Thailand. Southeast Asian J.Trop.Med.Public Health31(Suppl 1) 111-118.

42. Andersson, M.B., Ketterman, A.J., and Bogoyevitch, M.A. (1998) Differential regulation of parallel mitogen-activated protein kinases in cardiac myocytes revealed by phosphatase inhibition. Biochem.Biophys. Res. Commun. 251:328-333.

43. Foncea, R., Andersson, M., Ketterman, A., Blakesley, V., Sapag-Hagar, M., Sudgen, P.H., LeRoith, D. and Lavandero, S.  (1997) Insulin-like growth factor -1 rapidly activates multiple signal transduction pathways in cultured rat cardiac myocytes. J. Biol. Chem. 272:19115-19124.

44. Bogoyevitch, M.A., Gillespie-Brown, J., Ketterman, A.J., Fuller, S.J., Ben-Levy, R., Ashworth, A., Marshall, C.J. and Sugden, P.H. (1996) Stimulation of myocardial stress-activated protein kinases by ischaemia and ischaemia-reperfusion. Circ. Res.79: 162-173.

45. Prapanthadara, L., Koottathep, S., Promtet, N., Hemingway, J. and Ketterman*, A.J. (1996) Purification and characterization of a major glutathione S-transferase from the mosquito Anopheles dirus (species B). Insect Biochem. Molec. Biol.26: 277-285.

46. Bogoyevitch, M.A., Ketterman, A.J. and Sugden, P.H. (1995) Cellular stresses activate c-Jun N-terminal protein kinases in cultured ventricular myocytes. J. Biol. Chem.270: 29710-29717.

47. Prapanthadara, L., Hemingway, J. and Ketterman, A.J. (1995)  DDT-resistance in Anopheles gambiae Giles from Zanzibar Tanzania based on increased DDT-dehydrochlorinase activity of glutathione S-transferases. Bull. Entomol. Res.85: 267-274.

48. Jayawardena, K.G.I., Karunaratne, S.H.P.P., Ketterman, A.J. and Hemingway, J. (1994) Determination of the role of elevated B2 esterase in insecticide resistance in Culexquinquefasciatus (Diptera: Culicidae) from studies on the purified enzyme. Bull. Entomol. Res.84: 39-44.

49. Prapanthadara, L., Hemingway, J. and Ketterman*, A.J. (1993) Partial purification and characterization of glutathione S-transferases involved in DDT resistance from the mosquito Anopheles gambiae. Pestic. Biochem. Physiol.47: 119-133.

50. Ketterman*, A.J., Karunaratne, S.H.P.P., Jayawardena, K.G.I., and Hemingway, J. (1993) Qualitative differences between populations of Culexquinquefasciatus in both the esterase A2 and B2 which are involved in insecticide resistance. Pestic. Biochem. Physiol.47: 142-148.

51. Karunaratne, S.H.P.P., Jayawardena, K.G.I., Hemingway, J. and Ketterman*, A.J. (1993) Characterization of a B type esterase involved in insecticide resistance from the mosquito Culexquinquefasciatus. Biochem. J.294: 575-579.

52. Ketterman*, A.J., Jayawardena, K.G.I. and Hemingway, J. (1992) Purification and characterisation of a carboxylesterase involved in insecticide resistance from the mosquito Culexquinquefasciatus. Biochem. J.287: 355-360.

53. Ketterman*, A.J., Bowles, M.R. and Pond, S.M. (1989) Purification and characterisation of two human liver carboxylesterases. Int. J. Biochem.21: 1303-1312.

54. Ketterman*, A.J., Pond, S.M. and Becker, C.E. (1987) The effects of differential induction of cytochrome P-450, carboxylesterase and glutathione S-transferase activities on malathion toxicity in mice. Toxicol. Appl. Pharmacol.87: 389 392.

55. Hammock, B.D., Loury, D.N., Moody, D.E., Ruebner, B., Baselt, R., Milam, K.M., Volberding, P., Ketterman, A. and Talcott, R. (1984) A methodology for the analysis of the preneoplastic antigen. Carcinogenesis5: 1467-1473.

56. Talcott, R.E., Pond, S.M., Ketterman, A. and Becker, C.E. (1984) Diethylsuccinatecarboxylesterase activity as an indicator of liver damage. IRCS Med. Sci.12: 616 617.

57. Talcott, R.E., Ketterman*, A. and Giannini, D.G. (1984) Inactivation of microsomal NADPH-cytochrome C reductase by sulfhydryl-reactive derivatives of menadione. Biochem. Pharmacol.33: 2663-2668.

58. Lotti, M., Ketterman, A., Waskell, L. and Talcott, R.E. (1983) Meperidinecarboxylesterase in mouse and human livers. Biochem. Pharmacol.32: 3735-3738.

59. Talcott, R.E., Pond, S.M., Ketterman, A. and Becker, C.E. (1982) Ethylesterases as indicators of liver damage.  I. Studies on malathioncarboxylesterases. Toxicol. Appl. Pharmacol.65: 69 74.

60. Mallipudi, N.M., Talcott, R.E., Ketterman, A. and Fukuto, T.R. (1980) Properties and inhibition of rat malathioncarboxylesterases. J. Toxicol. Environ. Health, 6: 585 596.

61. Talcott, R., Ketterman, A., Harger, W., Denk, H., Kerjaschki, D., Zeiler, I. and Eckerstorfer, R. (1980) Microsomal lipid peroxidation: catalysis, effects and inhibition by cytosolic protein. InMicrosomes, Drug Oxidations and Chemical Carcinogens (Proceedings of the 4th International Symposium on Microsomes and Drug Oxidations), pp. 753-759, Academic Press, New York.

62. Hammock, B.D., Kuwano, E., Ketterman, A. Scheffrahn, R.H., Thompson, S.N. and Sallume, D. (1978) Acute toxicity and development effects of analogues of ethyl 1-(4-chlorophenoxy)-1-methylpropionate on two insects, Oncopeltusfasciatus and Tenebriomolitor. J. Agric. Food Chem.26: 166-170.

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