Kakanang Buranaamnuay

Kakanang Buranaamnuay, Ph.D.

Ph.D. (Theriogenology), Chulalongkorn University, Thailand, 2009
Ext. 1389
Email:  kakanang.bur@mahidol.ac.th
Field of Research: Animal sperm preservation and evaluation of sperm quality

My current research, the study on factors affecting the quality of cooled and frozen-thawed sperm in many species of animals such as dairy cattle, pig, domestic cat and even Asian elephant (Elephas maximus), ultimately aims at finding the suitable sperm preservation protocol which reveals the good sperm quality.

Generally, in vitro preservation of sperm cells could be accomplished by two methods, i.e. cooling, at the temperature lower than body temp but higher than the freezing point of water, and freezing, at subzero temperature. The important advantage of freezing over cooling is the longer preservation time of sperm which makes frozen sperm suitable to be used in the distant area especially across country as well as used to preserve gametes of superior genetic animals as a sperm/semen bank. Nevertheless, irrespective of species of animal, the in vitro and in vivo qualities of sperm after being frozen-thawed are usually decreased hugely in comparison to those of fresh and cooled sperm. This is one of the reasons for the significance of doing more researches on various aspects of animal sperm.

Selected Publications

1. *Buranaamnuay, K. Sperm-TALP: an alternative extender for retrieving and diluting epididymal sperm in the domestic cat. Reprod Dom Anim. 2013. 48: 912-917.

2. *Buranaamnuay, K., Mahasawangkul, S. and Saikhun K. The in vitro quality of frozen-thawed Asian elephant (Elephas maximus) spermatozoa in semen supplemented with Equex STM paste and oxytocin during and after cryopreservation. Reprod Biol. 2013. 13: 169-171.

3. *Buranaamnuay, K., Grossfeld, R., Struckmann, C. and Rath, D. Influence of cryoprotectants glycerol and amides, combined with antioxidants on quality of frozen-thawed boar sperm. Anim Reprod Sci. 2011. 127: 56-61.

4. *Buranaamnuay, K., Tummaruk, P., Singlor, J., Rodriguez-Martinez, H. and Techakumphu, M.  Effects of straw volume and Equex-STM® on boar semen quality after cryopreservation. Reprod Dom Anim. 2009. 44: 69-73.

5. Tejerina, F., Buranaamnuay, K., Saravia, F., Wallgren, M. and *Rodriguez-Martinez, H. Assessment of motility of ejaculated, liquid-stored boar spermatozoa using computerized instruments. Theriogenology 2008.  69: 1129-1138.

Full Publications

1. *Buranaamnuay, K. Sperm-TALP: an alternative extender for retrieving and diluting epididymal sperm in the domestic cat. Reprod Dom Anim. 2013. 48: 912-917.

2.  *Buranaamnuay, K., Mahasawangkul, S. and Saikhun K. The in vitro quality of frozen-thawed Asian elephant (Elephas maximus) spermatozoa in semen supplemented with Equex STM paste and oxytocin during and after cryopreservation. Reprod Biol. 2013. 13: 169-171.
3. Wongkaweewit, K., Prommachart, P., Raksasub, R., Buranaamnuay, K., Techakumphu, M., De Rensis, F. and *Tummaruk, P. Effect of the administration of GnRH or hCG on time of ovulation and the onset of estrus-to-ovulation interval in sows in Thailand. Trop Anim Health Prod. 2012. 44: 467-470.
4. *Buranaamnuay, K., Grossfeld, R., Struckmann, C. and Rath, D. Influence of cryoprotectants glycerol and amides, combined with antioxidants on quality of frozen-thawed boar sperm. Anim Reprod Sci. 2011. 127: 56-61.
5. *Buranaamnuay, K., Tummaruk, P. and Techakumphu, M. Intra-uterine insemination with low numbers of frozen-thawed boar spermatozoa in spontaneous and induced ovulating sows under field conditions. Livestock Science 2010. 131: 115-118.
6. *Buranaamnuay, K., Wongtawan, T, Masuwattana, S, Tummaruk, P. and Techakumphu, M. Intra-uterine and deep intra-uterine insemination using cryopreserved boar semen in spontaneously-ovulating sows. Thai J Vet Med. 2010. 40 (2): 215-219.
7. Tretipskul, C., Buranaamnuay, K., Koonjaenak, S., Tummaruk, P. and *Techakumphu, M.  The use of computer-assisted sperm analysis (CASA) for discriminating a series of motility pattern of frozen-thawed boar semen. Thai J Vet Med. 2010. 40 (1): 25-30.
8. *Buranaamnuay, K., Tummaruk, P., Singlor, J., Rodriguez-Martinez, H. and Techakumphu, M.  Effects of straw volume and Equex-STM® on boar semen quality after cryopreservation. Reprod Dom Anim. 2009. 44: 69-73.
9. Tejerina, F., Buranaamnuay, K., Saravia, F., Wallgren, M. and *Rodriguez-Martinez, H. Assessment of motility of ejaculated, liquid-stored boar spermatozoa using computerized instruments. Theriogenology 2008. 69: 1129-1138.
10. *Buranaamnuay, K., Singlor, J., Tummaruk, P. and Techakumphu, M. The establishment of boar semen cryopreservation in Thailand: post-thaw semen quality, sperm concentration and variation among ejaculates. Thai J Agri Sci. 2008. 41(3-4): 135-141.
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