Sakol Panyim, Ph.D.,
|Ph.D. (Biochemistry), University of Iowa, 1971
Field of Research: Shrimp Molecular Biology
Shrimp culture industry (approximately 80 billion Baht value) is greatly affected byloss (as much as 25%) due to viral diseases. Raising protective immunity in cultured shrimpis a strategic research to find a means to prevent the economic loss of the shrimp industry.However, the protective immunity in shrimp is different from that in human and is largelyunknown. A new approach to raise protective immunity in shrimp is desirable. A method toprevent pathogenic virus to replicate inside shrimp cells is a promising alternative to protectshrimps from viral diseases. Gene silencing by small interference RNA (siRNA) has beendemonstrated to inhibit viral replication in a few invertebrate cells such as those ofDrosophila. Thus, it is envisaged that if gene silencing by siRNA was functional in shrimpcells it should lead to a devise of siRNA which could inactivate the viral mRNA andconsequently inhibit the viral replication. A failure of the viruses to replicate in shrimp shouldgive rise to a new means of protective immunity. Specific siRNA may be designed tosynthesize inside shrimp cells. One method is to use a “friendly virus” of shrimp as a vectorto deliver siRNA. This engineered viral vector may be further developed to create a new typeof vaccine in shrimp and other animals.
1. Plongthongkum, N., Kullawong, N., Panyim, S. and Tirasophon, W. (2007) Ire1 regulated XBP1 mRNA splicing in essential for the unfolded protein response (UPR) in Drosophila melangaster. Biochem. Biophy. Res. Comm. 354:789-794.
2. Tirasophon W, Yodmuang S, Chinnirunvong W, Plongthongkum N, Panyim, S. (2007)Therapeutic inhibition of yellow head virus multiplication in infected shrimps by YHV-protease dsRNA. Antiviral Research 74(2):150-155.
3. Yodmuang, S., Tirasophon, W., Roshorm, Y., Chinnirunwong, W. and Panyim, S. (2006) YHVprotease dsRNA inhibits YHV replication in Penaeus monodon and prevents mortality. Biochem. Biophy. Res. Comm. 341:351-356.
4. Treerattrakool S., Udomkit A. and Pamyim S. (2006) Anti-CHH antibody causes impairedhyperglycemia in Penaeus monodon. J. Biochem. Mol. Biol. 39, 371-376.
5. Sukhumsirichart, W. Attasart, P. Boonsaeng, V. Panyim, S. (2006) Complete nucleotidesequence and genomic organization of hepatopancreatic parvovirus (HPV) of Penaeusmonodon. Virology 346(2):266-77.