Wipawan Thangnipon

Wipawan Thangnipon, Ph.D.,
Assoc. Prof.

 

Wipawan2Ph.D. (Neurochemistry), University of London, UK, 1985
Email:  wipawan.tha@mahidol.ac.th
Tel.1203
Field of Research: Neurobiology of Alzheimer’s disease

In our laboratory we study the mechanisms causing neuronal degeneration in Alzheimer’s disease (AD) and thedevelopment of Thai medicinal plants for pharmaceutical therapy in AD. The initial approach involved the reduction of calcium-binding proteins in the AD brain. We have developed a system called a rapid and non-isotopic chain reaction-single strand conformation polymorphism (PCR-SSCP) method for determining the distribution of APOE genotypes in AD patients. This study confirmed that the APOE ε4 allele is a risk factor in Thai AD patients, whereas the APOE ε2 allele is commonly found in an age-matched subjects. In cell culture models, our findings show that β-amyloid induces neuronal death through the p75 neurotrophin receptor. In parallel, we have been investigating Thai medicinal plants for therapeutic use. As expected our findings show that N-trans-feruloyltyramine, an antioxidant, purified from Polyalthia suberosa has protective effects against β-amyloid-induced neurotoxicity and oxidative stress in cortical cell cultures. In human neural stem cells (hNSC), current studies are investigating the mechanisms of the genetically modified hNSC secreting neurotrophins such as BDNF, GDNF, NT-3 and IGF-I. Those cells can protect neuronal death and enhance ChAT expression when they co-cultured with rat septal cells treated with β-amyloid. In our previous studies, we demonstrated that N-benzylcinnamide (PT-3) purified from Piper submultinerve can prevent Aβ neurotoxicity via the reduction of ROS production, suppression of apoptosis, attenuation of inflammation, and downregulation of stress-activated kinases. PT-3 also exerts protective effects on the aged rat cortex and hippocampus. Recently, stem cells have been actively investigated in our laboratory and they have shown some benefits to replace the neuronal loss in AD patients.

Selected Publications

1. Suwanna, N, *Thangnipon, W., Soi-ampornkul, R. (2014) Neuroprotective effects of diarylpropionitrile against β-amyloid peptide-induced neurotoxicity in rat cultured cortical neurons. Neurosci Lett. 2014; 578: 44-9.

2. *Thangnipon W, Puangmalai N, Chinchalongporn V, Jantrachotechatchawan C, Kitiyanant N, Soi-ampornkul R, Tuchinda P, Nobsathian S.  N-benzylcinnamide protects rat cultured cortical neurons from β-amyloid peptide-induced neurotoxicity. Neurosci Lett 2013; 556: 20-5.

3. *Thangnipon W,  Suwanna N, Kitiyanant N,  Soi- ampornkul R,Tuchinda P, Munyoo B, Nobsathian S.  Protective role of N-trans-feruloyltyramine against β-amyloid peptide-induced neurotoxicity in rat cultured cortical neurons. Neurosci Lett 2012; 513: 229-32.

4. Kitiyanant N, Kitiyanant Y, Svendsen CN, *Thangnipon W. BDNF-, IGF-1- and GDNF-secreting human neural progenitor cells rescue amyloid  β–induced toxicity  in cultured  rat  septal neurons. Neurochem Res 2012; 37: 143-52.

5. Sotthibundhu A, Sykes AM, Fox B, Underwood CK, *Thangnipon W, Coulson EJ. β-Amyloid1–42 induces neuronal death through the p75 neurotrophin receptor. J Neurosci 2008; 28: 3941–6.

 

Full Publications

1. Suwanna, N., *Thangnipon, W., Soi-ampornkul R. (2014). Neuroprotective effects of diarylpropionitrile against β-amyloid peptide-induced neurotoxicity in rat cultured cortical neurons. Neurosci Lett. 578, 44-9.

2. *Thangnipon, W., Puangmalai, N., Chinchalongporn, V., Jantrachotechatchawan, C., Kitiyanant, N., Soi-ampornkul, R., Tuchinda, P., Nobsathian, S. (2013).  N-benzylcinnamide protects rat cultured cortical neurons from β-amyloid peptide-induced neurotoxicity. Neurosci Lett. 556, 20-5.

3. *Thangnipon, W.,  Suwanna, N., Kitiyanant, N.,  Soi- ampornkul, R.,Tuchinda, P., Munyoo, B., Nobsathian, S. (2012). Protective role of N-trans-feruloyltyramine against β-amyloid peptide- induced neurotoxicity in rat cultured cortical neurons. Neurosci Lett. 513, 229-32.

4. Kitiyanant N, Kitiyanant Y, Svendsen CN, *Thangnipon W. BDNF-, IGF-1- and GDNF-secreting human neural progenitor cells rescue amyloid  β–induced toxicity  in cultured  rat  septal neurons. Neurochem Res 2012; 37: 143-52.

5. Laohawattanakun, J., Chearskul, S., Dumrongphol, H.,  Jutapakdeegul, N., Yensukjai, J., Khumphan, N., Niltiean, S., *Thangnipon, W. (2011). Influence of music training on academic examination-induced stress in Thai adolescents. Neurosci Lett. 487, 310-12.

6. Buntup, D., Chayasadom, A., Surarit, R.,Jutapakdeegul, N., *Thangnipon, W. (2009). Effects   of amyloid-β peptide on glutamine transporter mRNA expression and cell viability in cultured rat cortical cells. ScienceAsia. 35, 156-60.

7. Sotthibundhu, A., Li, Q., Thangnipon, W. and *Coulson, E.J. (2009). Aβ 1–42 stimulates adult SVZ neurogenesis through the p75 neurotrophin receptor. Neurobiology of Aging. 30, 1975-85.

8. Sotthibundhu, A., Sykes, A.M., Fox, B., Underwood, C.K., Thangnipon, W., *Coulson, E.J. (2008). β-Amyloid1–42 Induces Neuronal Death through the p75 Neurotrophin Receptor. Journal of Neuroscience 28, 3941–3946.

9. Sotthibundhu A, Buntup D, Sanghirun C, Cherdchu K, Cheeramakara C, ChansirikarnjanS, Nimkulrat E, Udommongkol C, Wongmek W, *Thangnipon W (2008). Low SerumVitamin B12 in Alzheimer’s Patients as Detected by a Solid Phase Radioimmunoassay. Siriraj Med J. 60, 66-68.

10. *Tong, L., Balazs, R., Soiampornkul, R., Thangnipon, W., Cotman, C.W. (2008). Interleukin-1β impairs brain derived neurotrophic factor-induced signal transduction. Neurobiol Aging. 29, 1380-1393.

11. Buntup, D., Skare, Ø., Solbu, T.T., Chaudhry, F.A., *Storm-Mathisen, J., *Thangnipon, W. (2008). β-Amyloid 25-35 Peptide Reduces the Expression of Glutamine Transporter SAT1in Cultured Cortical Neurons. Neurochem Res 33, 248–256.

12. Soiampornkul, R., *Tong, L., Thangnipon, W., Balazs, R., Cotman, C.W. (2008). Interleukin-1β interferes with signal transduction induced by neurotrophin-3 in corticalneuron. Brain Res. 1188, 189-97.

13. Kamruecha, W., Chansirikarnjana, S., Nimkulrat, E., Udommongkol, C., Wongmek , W., *Thangnipon, W. (2006). Rapid detection of apolipoprotein E genotypes in Alzheimer’s disease using polymerase chain reaction-single strand conformation polymorphism. Southeast Asian J Trop Med Public Heath 37, 793-7.

14. Soi-ampornkul, R., Tong, L., *Thangnipon, W., Cotman, C., Balazs, R. (2005). Impairment of neurotrophin-3-induced phosphorylation of cAMP response elementbinding proteincaused by interleukin-1β in cultured cortical neurons: a model of Alzheimer’s disease. ScienceAsia. 32, 201-5.

15. Put-ti-noi, S., Petmitr, S., Chanyavanich, V., Sangruji, T., Theerapancharoen, V., Hayashi, K., *Thangnipon, W. (2004). Chromosome 10 and 17 deletions and p53 gene mutations in Thai patients with astrocytomas. Oncol Rep. 11, 207-12.

16. Buntup, D., Petmitr, S., Wongkajornsilp, A., Chanyavanich, V., Sangruji, T., Theerapancharoen, V., Kotchabhadi, N., Jutapakdeegul, N., Hayashi, K., *Thangnipon, W. (2002). Mutational analysis of the PTEN gene localized at chromosome 10q23 in Thai patients with gliomas. Science Asia 28, 115-118.

17. Chawengchao, B., Petmitr, S., Ponglikitmongkol, M., Chanyavanich, V., Sangruji, T., Theerapuncharoen, V., Hayashi, K., *Thangnipon, W. (2001). Detection of a novel point mutation in the p53 gene in grade II astrocytomas by PCR-SSCP analysis with additional Klenow treatment. Anticancer Res. 21, 2739-44.

18. *Thangnipon, W., Mizoguchi, M., Kukita, Y., Inazuka, M., Iwaki, T., Fukui, M., Hayashi, K. (1999). Distinct pattern of PCR-SSCP analysis of p53 mutations in human astrocytomas. Cancer Lett. 141, 195-201.

19. *Thangnipon, W. (1998) Alterations of calcium-binding proteins in Alzheimer’s disease and hippocampal cell cultures. J Neurochem. 70, S27.

20. *Thangnipon, W., Thangnipon, W., Luangpaiboon, P., Chinabut, S. (1995) Effects of the organophosphate, monocrotophos, on acetylcholinesterase activity in the nile tilapia fish (Oreochromis niloticus) brain. Neurochem Res. 20, 587-591.

21. *Thangnipon, W., Haruthaithanasan, P., Phanviriyapong, S, Thangnipon W., Surarit, R. (1994). Effects of organophosphate and carbamate insecticides on the nervous system in Thai farmers. Mahidol Univ J. 1, 6-14. (Abstract in English)

22. *Thangnipon, W., Iwamoto, N., Emson, F.C. (1992). Rapid purification of a glycoprotein specifically associated with a population of cerebral cortical interneurones which degenerate in Alzheimer’s disease. Thai J Physiol Sci 5, 11-6.

23. *Thangnipon, W., Iwamoto, N., Kiyama, H., Faull, R.L.M., Emson, P.C. (1992). Reduction of calbindin mRNA expression in the temporal cortex in Alzheimer’s disease. J Sci Soc Thailand 18, 121-8.

24. Thangnipon, W., *Balazs, R. (1992) Developmental changes in gangliosides in cultured cerebellar granule neurones. Neurochem Res. 17, 45-59

25. Iwamoto, N., Thangnipon, W., Crawford, C., *Emson, P.C (1991) Localization of calpain immunoreactivity in senile plaques and in neurones undergoing neurofibrillary degeneration in Alzheimer’s disease. Brain Res. 561, 177-80.

26. *Liu, D., Thangnipon, W., McAdoo, D.J. (1991). Excitatory amino acids rise to toxic level upon impact injury to the rat spinal cord. Brain Res. 547, 344-8.

27. Levi, G., Aloisi, F., Ciotti, M.T., Thangnipon, W., Kingsbury, A., *Balazs, R. (1989). Preparation of 98% pure cerebellar granule cell cultures. In: A Dissection and Tissue Culture Manual of the Nervous System, Eds. Shahar, A., de Vellis, J., Vernadakis, A. and Haber, B., 211-4.

28. *Balazs, R., Gallo, V., Kingsbury, A., Thangnipon, W., Smith R., Atterwill, C., Woodhams, P. (1987). Factors affecting the survival and maturation of nerve cells in culture. In. Glia-Neuronal Communication in Developmental Regeneration, Eds. Althaus, H.H. and Seifert, W., 287-302.

29. Thangnipon, W., Kingsbury, A., * Webb, M., Balazs, R.(1983). Observation on rat cerebellar cells in vitro: relationship between neurons and astrocytes. Develop. Brain Res. 11, 177-89.

30. Thangnipon, W., Taxt, T., Brodal, P., *Storm-Mathisen, J. (1983). The corticopontine projection: Axotomy induced loss of high affinity L-glutamate decarboxylase or choline acetyltransferase, in the pontine nuclei. Neuroscience 8, 449-457.

31. Thangnipon, W., *Storm-Mathisen, J. (1981). K+-Evoked Ca2+-dependent release of D-[3H] aspartate from terminals of the corticopontine pathway. Neurosci Lett 23,181-185.

32. Thangnipon, W., *Chulavatnatol, M. (1979). Autoproteolysis in human seminal plasma under acidic condition. Int J Fertil. 24, 260-263.

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